无机铅对大鼠脑线粒体呼吸及能量产生的影响。
Effect of inorganic lead on rat brain mitochondrial respiration and energy production.
作者信息
Gmerek D E, McCafferty M R, O'Neill K J, Melamed B R, O'Neill J J
出版信息
J Neurochem. 1981 Mar;36(3):1109-13. doi: 10.1111/j.1471-4159.1981.tb01706.x.
Toxicologically significant amounts of inorganic lead were added to rat brain mitochondrial preparations that did not contain EDTA or Pi. The binding of the lead to the mitochondria was measured by anodic stripping voltometry. In the presence of lead, the respiratory control ratios decreased, implying a decrease in the degree of dependence of respiration on a phosphate acceptor. Nucleotide contents were also measured, and in the presence of inorganic lead the actual amounts of ATP formed from ADP were found to be significantly decreased as well.
将具有毒理学意义的无机铅添加到不含乙二胺四乙酸（EDTA）或无机磷酸盐（Pi）的大鼠脑线粒体制剂中。通过阳极溶出伏安法测量铅与线粒体的结合情况。在有铅存在的情况下，呼吸控制率降低，这意味着呼吸对磷酸盐受体的依赖程度降低。同时也测量了核苷酸含量，发现在有无机铅存在的情况下，由二磷酸腺苷（ADP）形成的三磷酸腺苷（ATP）的实际量也显著减少。
Zotero 插件