人膀胱和肺癌细胞系的转化基因与哈维氏和克里斯滕氏肉瘤病毒的ras基因同源。

Der C J, Krontiris T G, Cooper G M

Proc Natl Acad Sci U S A. 1982 Jun;79(11):3637-40. doi: 10.1073/pnas.79.11.3637.

Blot hybridization analysis indicated that NIH 3T3 mouse bladder transformed by high molecular weight DNAs of a human bladder and a human lung carcinoma cell line contained new sequences homologous, respectively, to the transforming genes of Harvey (rasH) and Kirsten (rasK) sarcoma viruses. The unique ras sequences were present in multiple independent NIH cell lines transformed in both primary and secondary transfection assays and corresponded to ras sequences normally present in human DNAs. The ras gene product was expressed in NIH cells transformed by bladder carcinoma DNAs and in the human bladder carcinoma cell lines at levels 2- to 4-fold greater than the level observed in nontransformed NIH 3T3 cells. These results indicate that the transforming genes of these human tumor cell lines are the cellular homologs of two retroviral transforming genes.

印迹杂交分析表明，用人膀胱癌和人肺癌细胞系的高分子量DNA转化的NIH 3T3小鼠膀胱中分别含有与哈维（rasH）和柯尔斯滕（rasK）肉瘤病毒的转化基因同源的新序列。独特的ras序列存在于在初次和二次转染试验中转化的多个独立的NIH细胞系中，并且与人类DNA中正常存在的ras序列相对应。ras基因产物在由膀胱癌DNA转化的NIH细胞以及人膀胱癌细胞系中的表达水平比在未转化的NIH 3T3细胞中观察到的水平高2至4倍。这些结果表明，这些人类肿瘤细胞系的转化基因是两种逆转录病毒转化基因的细胞同源物。