Donahue J P, Thompson H A
J Bacteriol. 1981 May;146(2):808-12. doi: 10.1128/jb.146.2.808-812.1981.
The addition of coliphage Q beta ribonucleic acid to a cell-free translation system obtained from Coxiella burnetii cells caused the formation of monosomes and disomes from ribosomal subunits and resulted in synthesis of viral coat protein. Rickettsial ribosomes and associated translation factors functioned together, with fidelity, efficiency, and a specificity similar to those of free-living gram-negative bacteria.
向从伯氏考克斯氏体细胞获得的无细胞翻译系统中添加噬菌体Qβ核糖核酸,会导致核糖体亚基形成单体和二聚体,并引发病毒外壳蛋白的合成。立克次氏体核糖体及相关翻译因子协同发挥作用,其保真度、效率和特异性与自由生活的革兰氏阴性菌相似。