Sanderson M J, Sleigh M A
Pediatr Res. 1981 Mar;15(3):219-28. doi: 10.1203/00006450-198103000-00005.
A quantifiable assay is described in which the lateral cilia of the sea mussel Mytilus edulis are used to examine the effects of serum on ciliary function. Human sera and sera from a variety of animals have been found to induce a change in ciliary metachrony. This alteration in activity appears to be brought about by the agglutination of adjacent cilia into blocks, with the effect of increasing the wavelength; the beat frequency remains steady. Parallel studies with the rabbit cilia assay have shown the agglutination of tracheal cilia into clumps by adult bovine serum. The dilution of adult bovine serum lengthens the time before dyskinesia is first observed in both the Mytilus and rabbit assays. Fetal bovine or human cord sera do not cause ciliary dyskinesia or ciliary agglutination. The evidence presented shows that the observed ciliary dyskinesia can be induced by sera from a variety of animals and is not specific to cystic fibrosis; it also suggests that the serum immunoglobulin fraction is responsible for these effects--initial studies implicate IgM.
本文描述了一种可量化的检测方法，其中利用海贻贝紫贻贝的外侧纤毛来检测血清对纤毛功能的影响。已发现人血清和多种动物的血清会引起纤毛运动同步性的变化。这种活性改变似乎是由于相邻纤毛凝集形成块状物，从而增加了波长；搏动频率保持稳定。用兔纤毛检测法进行的平行研究表明，成年牛血清会使气管纤毛凝集形成团块。在紫贻贝和兔检测中，成年牛血清的稀释会延长首次观察到运动障碍的时间。胎牛血清或人脐带血清不会导致纤毛运动障碍或纤毛凝集。所提供的证据表明，观察到的纤毛运动障碍可由多种动物的血清诱导，并非囊性纤维化所特有；这也表明血清免疫球蛋白部分是造成这些影响的原因——初步研究表明是IgM。
