Womack J E, Cramer D V
Biochem Genet. 1980 Oct;18(9-10):1019-26. doi: 10.1007/BF00500132.
Starch gel electrophoresis and histochemical staining with L-leucyl-L-tyrosine have revealed genetic variation for dipeptidase in Rattus norvegicus. The tissue distribution, substrate specificity, and heterozygous expression as a monmeric protein suggest homology of the variant peptidase to human PEP-C and mouse Pep-3 (Dip-1). We propose Peptidase-3 (Pep-3) as a name for this autosomal locus in the rat. The allele responsible for slower (less anodal) electrophoretic migration is designated Pep-3a and is characteristic of strain ACI/Pit. A faster (more anodal) electrophoretic mobility is the product of the Pep-3b allele in strain F344/Pit. Twenty-five additional inbred strains carry Pep-3a and 16 others carry Pep-3b. Wild rats trapped in Pittsburgh were polymorphic for this locus. Alleles at Pep-3 segregated independently of c (linkage group I), a (linkage group IV), RT2 and Es-1 (linkage group V), h (linkage group VI), and RTI (linkage group VIII).
淀粉凝胶电泳和用L-亮氨酰-L-酪氨酸进行组织化学染色揭示了褐家鼠中二肽酶的遗传变异。该变异肽酶的组织分布、底物特异性以及作为单体蛋白的杂合表达表明其与人类PEP-C和小鼠Pep-3（Dip-1）具有同源性。我们提议将大鼠中这个常染色体位点命名为肽酶-3（Pep-3）。导致电泳迁移较慢（向阳极移动较少）的等位基因被命名为Pep-3a，是ACI/Pit品系的特征。电泳迁移较快（向阳极移动较多）是F344/Pit品系中Pep-3b等位基因的产物。另外25个近交系携带Pep-3a，另外16个携带Pep-3b。在匹兹堡捕获的野生大鼠在这个位点上具有多态性。Pep-3位点的等位基因与c（连锁群I）、a（连锁群IV）、RT2和Es-1（连锁群V）、h（连锁群VI）以及RTI（连锁群VIII）独立分离。
