Fleischmajer R, Perlish J S, Krieg T, Timpl R
J Invest Dermatol. 1981 May;76(5):400-3. doi: 10.1111/1523-1747.ep12520933.
Primary cultures of fibroblasts obtained from the papillary, reticular and subcutaneous layer of scleroderma skin were analyzed for protein synthesis by metabolic labeling and radioimmunoassays. Several of these cultures showed a 10- to 20-fold increase in the production of total protein and collagen as well as of fibronectin and type III procollagen as compared to cells from unaffected individuals. Most of the increases were noted in the reticular and subcutaneous layers. With cells from other patients increased synthesis was found in some of the explants or for only some of the products. The heterogeneity observed here could represent heterogeneity in the disease, in the cells studied or in the state of the disease at the time the cells were obtained.
通过代谢标记和放射免疫测定法，对取自硬皮病皮肤乳头层、网状层和皮下层的成纤维细胞原代培养物进行蛋白质合成分析。与未受影响个体的细胞相比，其中几种培养物的总蛋白、胶原蛋白、纤连蛋白和III型前胶原的产量增加了10至20倍。大部分增加出现在网状层和皮下层。对于其他患者的细胞，在一些外植体中或仅对某些产物发现合成增加。这里观察到的异质性可能代表疾病本身的异质性、所研究细胞的异质性或获取细胞时疾病的状态异质性。
