Haigwood N L, Jahn C L, Hutchison C A, Edgell M H
Nucleic Acids Res. 1981 Mar 11;9(5):1133-50. doi: 10.1093/nar/9.5.1133.
Three different repeat sequences have been mapped within the cloned EcoRI fragments that contain the adult beta-globin genes from the BALB/c (Hddd) mouse. One sequence, "a", occurs 1.5-2 kb 3' to the beta-major gene. A second, "b", is found 4kb 5' and 7.5kb 3' to the beta-minor gene. The 14kb EcoRI fragment bearing the beta-minor gene carries at least one additional repetitive element, "c". Probing a BALB/c DNA library with each repeat has demonstrated that these sequences are moderately to highly repetitive and are extensively interspersed with each other throughout the genome. In addition, repeats "a" and "b" are preferentially found in satellite and main-band DNa, respectively. The occurrence of these repeats elsewhere in the beta-globin cluster was demonstrated by probing the non-adult globin clones with each repeat. The arrangement of these repeats around the non-adult genes is 5'-"b"-"b"-epsilon y-beta hl-beta h2-"c"-beta h3-3'. Probing the C57BL/10 (Hbbs) adult gene clones with these repeats demonstrated that the distribution of these sequences in the adult region of these two haplotypes is essentially the same.
在克隆的包含BALB/c（Hddd）小鼠成年β-珠蛋白基因的EcoRI片段中，已定位出三种不同的重复序列。一种序列“a”位于β-主要基因下游1.5 - 2 kb处。第二种序列“b”在β-次要基因上游4 kb和下游7.5 kb处被发现。携带β-次要基因的14 kb EcoRI片段至少携带一个额外的重复元件“c”。用每种重复序列探测BALB/c DNA文库表明，这些序列具有中度到高度的重复性，并且在整个基因组中彼此广泛散布。此外，重复序列“a”和“b”分别优先存在于卫星DNA和主带DNA中。用每种重复序列探测非成年珠蛋白克隆证明了这些重复序列在β-珠蛋白基因簇其他位置的存在。这些重复序列在非成年基因周围的排列顺序是5'-“b”-“b”-εy-βh1-βh2-“c”-βh3-3'。用这些重复序列探测C57BL/10（Hbbs）成年基因克隆表明，这两种单倍型成年区域中这些序列的分布基本相同。
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