Shyman S, Weaver S
Nucleic Acids Res. 1985 Jul 25;13(14):5085-93. doi: 10.1093/nar/13.14.5085.
Two segments of DNA that have apparently inserted in the interval between the two adult beta-globin genes in BALB/c (Hbbd haplotype) but not in C57B1/10 (Hbbs haplotype) mouse strains have been described (1). These putative insertions, each about 1000 bp in length, mapped near a repetitive element. To determine the precise position of these alleged insertions, their target sites, and the nature of their boundaries, we cloned and sequenced the appropriate regions of both chromosomes. One of the two segments is not an insertion but rather a region between two independently integrated L1 repetitive elements (LINEs) (2), one in Hbbd and the other in the Hbbs chromosome. The other segment is an insertion of 940 bp which is located within the L1 element in the Hbbd chromosome. This insert is unusual in that it exists in only one copy in the BALB/c genome.
已经描述了两段DNA，它们明显插入到BALB/c（Hbbd单倍型）小鼠品系的两个成年β-珠蛋白基因之间的区间，但未插入到C57B1/10（Hbbs单倍型）小鼠品系中（1）。这些假定的插入片段，每个长度约为1000 bp，定位在一个重复元件附近。为了确定这些所谓插入片段的精确位置、它们的靶位点以及其边界的性质，我们克隆并测序了两条染色体的相应区域。两段中的一段不是插入片段，而是两个独立整合的L1重复元件（长散在核元件）之间的一个区域（2），一个在Hbbd染色体中，另一个在Hbbs染色体中。另一段是940 bp的插入片段，位于Hbbd染色体的L1元件内。这个插入片段不同寻常之处在于它在BALB/c基因组中仅以一个拷贝存在。
