Vierbuchen M, Klein P J, Uhlenbruck G, Klein H O, Schaefer H E, Fischer R
Recent Results Cancer Res. 1980;75:68-75. doi: 10.1007/978-3-642-81491-4_11.
Normal kidney tissue as well as hypernephromas were examined histochemically for the occurrence of lectin receptors. FITC- and rhodamine-labeled peanut agglutinin (PNA) and Ricinus communis agglutinin (RCA) were used for labeling different carbohydrate residues which may be of interest in the evaluation of the histogenesis of hypernephromas and possibly for concepts regarding the immunotherapy of these tumors. By using fluorescence microscopy, the receptor for PNA was found on the epithelial cells of the thin limb, distal convoluted tubules, and the collecting ducts. These binding sites occurred in a free as well as in a sialic-acid-substituted form and were mainly exposed on the luminal surface of the epithelial cells. They were absent, however, in the proximal convoluted tubules. This finding contrasts with the demonstration of RCA receptors in the brush border of these tubules. Moreover, RCA reacted with the epithelial cells of all tubules. The hypernephromas showed a wide range in the distribution and the quality of the lectin receptors, which were localized within the cytoplasm as well as in the cell membrane of the tumor cells. As demonstrated by our histochemical investigations, these kidney neoplasms may originate from any part of the tubules, and not only from the epithelial cells of the proximal convoluted tubules, as was suggested by earlier findings that employed antibodies against the brush-border antigens. In addition, the demonstration of sialic-acid-substituted and, in particular, of free PNA-receptors, which represent the immunodominant group of the Thomsen-Friedenreich antigen, may supply useful information on an immunotherapy concept.
对正常肾组织以及肾细胞癌进行组织化学检查，以检测凝集素受体的存在情况。使用异硫氰酸荧光素（FITC）和罗丹明标记的花生凝集素（PNA）以及蓖麻凝集素（RCA）来标记不同的碳水化合物残基，这对于评估肾细胞癌的组织发生可能具有重要意义，并且可能为这些肿瘤的免疫治疗提供相关概念。通过荧光显微镜观察发现，PNA受体存在于细段、远曲小管和集合管的上皮细胞上。这些结合位点以游离形式以及唾液酸取代形式存在，并且主要暴露于上皮细胞的管腔表面。然而，在近曲小管中不存在这些结合位点。这一发现与这些小管刷状缘中RCA受体的表现形成对比。此外，RCA与所有小管的上皮细胞都发生反应。肾细胞癌在凝集素受体的分布和性质方面表现出广泛的差异，这些受体定位于肿瘤细胞的细胞质以及细胞膜内。正如我们的组织化学研究所示，这些肾肿瘤可能起源于小管的任何部位，而不仅仅像早期使用抗刷状缘抗原抗体的研究所表明的那样起源于近曲小管的上皮细胞。此外，唾液酸取代的尤其是游离的PNA受体的显示，代表了Thomsen-Friedenreich抗原的免疫显性基团，这可能为免疫治疗概念提供有用信息。
