Raedler A, Boehle A, Otto U, Raedler E
J Urol. 1982 Nov;128(5):1109-13. doi: 10.1016/s0022-5347(17)53355-8.
We analyzed the outer cell membranes of normal kidney cells, hypernephroma cells, nude mouse transplants of hypernephroma tissue, and cell lines derived from hypernephroma using horseradish peroxidase-coupled lectins of different specificity, in order to investigate their binding to cryostat sections or the cell surfaces of suspended single cells. We showed that hypernephroma cells express higher amounts of receptors for Robinia pseudoacacia lectin, soy bean lectin and Phaseolus vulgaris lectin compared to those prepared from normal kidneys. In contrast, Helix pomatia lectin is bound to connective tissue elements of both malignant and non-malignant tissue exclusively. L-fucose and D-galactose residues are detectable on malignant cells, but not on their non-malignant counterparts. However, the expression of the latter varies considerably among cells from different hypernephromas. No significant difference could be demonstrated between hypernephroma, hypernephroma cell lines and hypernephroma transplants in regard to the carbohydrate profile on the cell surfaces.
我们使用具有不同特异性的辣根过氧化物酶偶联凝集素，分析了正常肾细胞、肾癌细胞、肾癌细胞裸鼠移植组织以及源自肾癌细胞系的外细胞膜，以研究它们与冷冻切片或悬浮单细胞表面的结合情况。我们发现，与正常肾脏制备的细胞相比，肾癌细胞表达更高量的刺槐凝集素、大豆凝集素和菜豆凝集素受体。相比之下，马蹄蟹凝集素仅与恶性和非恶性组织的结缔组织成分结合。恶性细胞上可检测到L-岩藻糖和D-半乳糖残基，而在其非恶性对应物上则未检测到。然而，后者的表达在来自不同肾肿瘤的细胞之间差异很大。在细胞表面的碳水化合物谱方面，肾肿瘤、肾癌细胞系和肾癌细胞移植之间未显示出显著差异。
