Kohler R B, Zimmerman S E, Wilson E, Allen S D, Edelstein P H, Wheat L J, White A
Ann Intern Med. 1981 May;94(5):601-5. doi: 10.7326/0003-4819-94-5-601.
Urine from nine patients with serogroup 1 Legionnaires' disease, 100 with infected or contaminated urine, 50 with bacteremic infections, 26 with infectious or infiltrative pulmonary diseases, and 65 with miscellaneous infectious or noninfectious illnesses, was examined by radioimmunoassay for the presence of Legionella pneumophila antigen(s). Urine of all nine Legionnaires' disease patients was clearly differentiated from that of the 241 control subjects by the assay. The antigen was detected from 2 to at least 10 days after the initiation of erythromycin therapy. A probable false-positive results occurred in a patient with necrotizing nosocomial pneumonia who died before definitive serologic evaluation for Legionnaires' disease could be completed. The antigen was stable at 100 degrees C for 30 minutes and was not degraded by trypsin. Its elution behavior suggested that its molecular weight is approximately 10 000. The radioimmunoassay appears to be a useful method for rapidly diagnosing serogroup 1 Legionnaires' disease.
对9例血清1型军团病患者、100例尿液受感染或被污染的患者、50例菌血症感染患者、26例感染性或浸润性肺部疾病患者以及65例患有其他感染性或非感染性疾病患者的尿液，采用放射免疫测定法检测嗜肺军团菌抗原的存在情况。通过该检测法，9例军团病患者的尿液与241例对照受试者的尿液明显区分开来。在开始使用红霉素治疗后的2至至少10天内检测到了该抗原。一名患有坏死性医院获得性肺炎的患者在完成军团病的确定性血清学评估之前死亡，出现了可能的假阳性结果。该抗原在100摄氏度下稳定30分钟，且不会被胰蛋白酶降解。其洗脱行为表明其分子量约为10000。放射免疫测定法似乎是快速诊断血清1型军团病的一种有用方法。
