厌氧生态系统中溶解氢的测量方法：在瘤胃中的应用
Method for measuring dissolved hydrogen in anaerobic ecosystems: application to the rumen.
作者信息
Robinson J A, Strayer R F, Tiedje J M
出版信息
Appl Environ Microbiol. 1981 Feb;41(2):545-8. doi: 10.1128/aem.41.2.545-548.1981.
Abstract
A method of transferring dissolved H2 to a CO2 headspace and then absorbing out the CO2 to concentrate the H2 before gas chromatographic analysis was developed to measure low concentrations of dissolved H2. A detection limit of 10 pmol of H2 ml-1 of water was achieved. When used ot monitor H2 changes in a bovine rumen, a 10-fold increase in H2 was noted 1 h after feeding and then declined rapidly to the normal steady-state concentration of 1 microM.
摘要
开发了一种将溶解的H2转移至CO2顶空，然后吸收除去CO2以在气相色谱分析前浓缩H2的方法，用于测量低浓度的溶解H2。实现了水中H2的检测限为10 pmol ml-1。当用于监测牛瘤胃中的H2变化时，喂食后1小时H2增加了10倍，然后迅速下降至1 microM的正常稳态浓度。
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