[亚硫酸氢钠与噬菌体DNP相互作用时DNA-蛋白质连接的形成]
[Formation of DNA-protein links upon interaction between sodium bisulfite and phage DNP].
作者信息
Skliadneva V B, Chekanovskaia L A, Khromov I S, Tikhonenko T I
出版信息
Biokhimiia. 1981 Mar;46(3):569-74.
PMID:7236809
Abstract
The conditions for linkage formation in phages Sd and P22 DNP under effects of sodium bisulfite were studied. Th linkage was estimated by the fall in phage infectiosity. After removal of the reagent excess prior to inoculation the modified phage was transferred to various buffers (12 salt systems). The correlation between infectiosity of preparations and changes in their CD spectra in corresponding calt solutions was established.
摘要
研究了在亚硫酸氢钠作用下噬菌体Sd和P22 DNP中连接形成的条件。通过噬菌体感染性的下降来估计连接情况。在接种前去除过量试剂后，将修饰后的噬菌体转移到各种缓冲液（12种盐体系）中。建立了制剂感染性与其在相应钙溶液中的圆二色光谱变化之间的相关性。
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