Tahara E, Ito H, Nakagami K, Shimamoto F
J Cancer Res Clin Oncol. 1981;100(1):1-12. doi: 10.1007/BF00405896.
A total of 30 inbred Wistar rats were orally administered 70 microgram/ml solution of N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) for 35 weeks and then tap water for the following 20 to 30 weeks. Four of the 20 females and two of ten males developed carcinoids in the glandular stomach, but no metastasis could be found. Carcinoids developed most frequently in the fundic portion along the greater curvature. Histologically, these tumors were medullary anaplastic carcinomas containing two different endocrine cell populations. The first cell type was argentaffin having the electron-dense, somewhat pleomorphic secretory granules (437-810 nm) and the second type was argyrophil having round granules with a dense core and a pale halo (550 nm). None of these tumors showed endocrine immunoactivity for gastrin, somatostatin, insulin, glucagon, and enkephalin. One of these gastric tumors developed into scirrhous carcinoma, but differentiated adenocarcinoma could not be seen in the glandular stomach.
总共30只近交系Wistar大鼠口服70微克/毫升的N-甲基-N'-硝基-N-亚硝基胍（MNNG）溶液35周，随后在接下来的20至30周给予自来水。20只雌性大鼠中有4只，10只雄性大鼠中有2只在腺胃发生类癌，但未发现转移。类癌最常发生在沿大弯的胃底部。组织学上，这些肿瘤是髓样间变性癌，含有两种不同的内分泌细胞群。第一种细胞类型是嗜银细胞，具有电子致密、 somewhat多形性的分泌颗粒（437 - 810纳米），第二种类型是亲银细胞，具有圆形颗粒，有致密核心和淡晕（550纳米）。这些肿瘤均未显示对胃泌素、生长抑素、胰岛素、胰高血糖素和脑啡肽的内分泌免疫活性。这些胃肿瘤中有一个发展为硬癌，但在腺胃中未见分化腺癌。
