Adler L T, Adler F L, Cohen C, Tissot R G, Lancki D
Transplantation. 1977 Nov;24(5):338-48. doi: 10.1097/00007890-197711000-00005.
In the course of experiments designed to demonstrate an active mechanism of allotype suppression in rabbits, spleen cells from adult donors were transferred to newborn recipients. Among 23 rabbits that received injections, 4 stable chimeras were formed, as determined by the production of serum immunoglobulins marked with light and heavy chain allotypes. The other rabbits that survived the immediate postinjection period displayed a temporary chimeric state lasting up to several weeks, after which they either succumbed to graft-versus-host disease or rejected the donor cells. One chimeric animal was apparently repopulated by the hemopoietic cells of the donor's spleen. Insofar as could be determined, the recipient's blood cells became phenotypically identical to those of the donor. This condition manifested itself as a loss of the recipient gene products associated with both lymphocyte and erythrocytes, accompanied by a seemingly total replacement with those of the donor.
在旨在证明兔同种异型抑制活性机制的实验过程中，将成年供体的脾细胞移植到新生受体体内。在接受注射的23只兔子中，通过产生带有轻链和重链同种异型标记的血清免疫球蛋白确定形成了4只稳定的嵌合体。在注射后急性期存活下来的其他兔子表现出持续数周的暂时嵌合状态，之后它们要么死于移植物抗宿主病，要么排斥供体细胞。一只嵌合动物显然被供体脾的造血细胞重新填充。就所能确定的而言，受体的血细胞在表型上变得与供体的血细胞相同。这种情况表现为与淋巴细胞和红细胞相关的受体基因产物的丧失，同时似乎完全被供体的基因产物所取代。
