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针对肺癌抗原的人源单克隆抗体。

Human monoclonal antibodies to lung-cancer antigens.

作者信息

Sikora K, Wright R

出版信息

Br J Cancer. 1981 May;43(5):696-700. doi: 10.1038/bjc.1981.101.

DOI:10.1038/bjc.1981.101
PMID:7248154
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2010695/
Abstract

Lymphocytes obtained from hilar and bronchial lymph nodes from 23 patients undergoing radical surgery for carcinoma of the bronchus were fused with established rat or mouse myeloma lines. 62% of the resultant hybrids were found to be secreting human Ig detected by a sensitive staphylococcal Protein A-coupled SRBC assay. Immunoglobulins synthesized by such hybrids were internally labelled with 3H-lysine and their antibody activity against a variety of membrane preparations determined. Nine monoclonal antibodies were found which bound to molecules on lung-cancer membranes and not on normal lung membranes from the same patient.

摘要

从23例接受支气管癌根治手术患者的肺门和支气管淋巴结中获取淋巴细胞，将其与已建立的大鼠或小鼠骨髓瘤细胞系进行融合。通过灵敏的葡萄球菌蛋白A偶联的绵羊红细胞试验检测发现，62%的所得杂交细胞分泌人免疫球蛋白。用3H-赖氨酸对这类杂交细胞合成的免疫球蛋白进行内标记，并测定其针对多种膜制剂的抗体活性。发现有9种单克隆抗体可与肺癌细胞膜上的分子结合，而不与同一患者正常肺细胞膜上的分子结合。

相似文献

1
Human monoclonal antibodies to lung-cancer antigens.针对肺癌抗原的人源单克隆抗体。
Br J Cancer. 1981 May;43(5):696-700. doi: 10.1038/bjc.1981.101.
2
Human monoclonal antibodies to glioma cells.针对胶质瘤细胞的人源单克隆抗体。
Br J Cancer. 1981 Jan;43(1):105-7. doi: 10.1038/bjc.1981.15.
3
Monoclonal antibodies and cell surface antigens.单克隆抗体与细胞表面抗原。
Cell Biol Int Rep. 1979 Jan;3(1):1-16. doi: 10.1016/0309-1651(79)90063-8.
4
In vitro production of human antibodies specifically reactive with human gastric cancer cells of established lines and autologous tissues.
J Surg Oncol. 1987 Nov;36(3):215-24. doi: 10.1002/jso.2930360313.
5
Detection of cell surface and intracellular antigens by human monoclonal antibodies. Hybrid cell lines derived from lymphocytes of patients with malignant melanoma.用人单克隆抗体检测细胞表面和细胞内抗原。源自恶性黑色素瘤患者淋巴细胞的杂交细胞系。
J Exp Med. 1983 Jul 1;158(1):53-65. doi: 10.1084/jem.158.1.53.
6
Monoclonal antibodies to human lung tumor antigens demonstrated by immunofluorescence and immunoprecipitation.通过免疫荧光和免疫沉淀法证实的针对人肺肿瘤抗原的单克隆抗体。
Cancer Res. 1982 Aug;42(8):3187-92.
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Antibodies from EB-virus-transformed lymphocytes of lymph nodes adjoining lung cancer.来自毗邻肺癌的淋巴结的EB病毒转化淋巴细胞的抗体。
Br J Cancer. 1982 Nov;46(5):802-5. doi: 10.1038/bjc.1982.273.
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Monoclonal xenogeneic antibodies to murine cell surface antigens: identification of novel leukocyte differentiation antigens.针对鼠细胞表面抗原的单克隆异种抗体：新型白细胞分化抗原的鉴定。
Eur J Immunol. 1978 Aug;8(8):539-51. doi: 10.1002/eji.1830080802.
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Generation of human monoclonal antibodies reactive with human mammary carcinoma cells.可与人乳腺癌细胞发生反应的人单克隆抗体的产生。
Proc Natl Acad Sci U S A. 1980 Nov;77(11):6841-5. doi: 10.1073/pnas.77.11.6841.
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A human monoclonal antibody directed to blood group i antigen: heterohybridoma between human lymphocytes from regional lymph nodes of a lung cancer patient and mouse myeloma.
J Immunol. 1986 Jun 1;136(11):4163-8.

引用本文的文献

1
Monoclonal antibodies against two human lung carcinoma cell lines.针对两种人肺癌细胞系的单克隆抗体。
Br J Cancer. 1982 Nov;46(5):794-801. doi: 10.1038/bjc.1982.272.
2
Loss of secretion in mouse-human hybrids need not be due to the loss of a structural gene.小鼠 - 人杂交细胞系中分泌功能的丧失不一定是由于结构基因的丢失。
J Exp Med. 1982 Nov 1;156(5):1380-9. doi: 10.1084/jem.156.5.1380.
3
Monoclonal antibodies in oncology.肿瘤学中的单克隆抗体
J Clin Pathol. 1982 Apr;35(4):369-75. doi: 10.1136/jcp.35.4.369.
4
UC 729-6, a human lymphoblastoid B-cell line useful for generating antibody-secreting human-human hybridomas.UC 729-6，一种可用于产生分泌抗体的人-人杂交瘤的人淋巴母细胞样B细胞系。
Proc Natl Acad Sci U S A. 1983 Oct;80(20):6327-31. doi: 10.1073/pnas.80.20.6327.
5
Human monoclonal antibodies: methods of production and some aspects of their application in oncology.人源单克隆抗体：生产方法及其在肿瘤学中的应用的一些方面。
Med Oncol Tumor Pharmacother. 1984;1(4):235-46. doi: 10.1007/BF02934529.
6
Monoclonal antibodies to human colon and colorectal carcinoma.
Br J Cancer. 1983 May;47(5):595-605. doi: 10.1038/bjc.1983.97.
7
Human hybridomas from patients with malignant disease.来自恶性疾病患者的人杂交瘤。
Br J Cancer. 1983 Jan;47(1):135-45. doi: 10.1038/bjc.1983.16.
8
Antibodies from EB-virus-transformed lymphocytes of lymph nodes adjoining lung cancer.来自毗邻肺癌的淋巴结的EB病毒转化淋巴细胞的抗体。
Br J Cancer. 1982 Nov;46(5):802-5. doi: 10.1038/bjc.1982.273.
9
Human tumor-associated antigens identified by monoclonal antibodies.通过单克隆抗体鉴定的人类肿瘤相关抗原。
Springer Semin Immunopathol. 1982;5(2):127-46. doi: 10.1007/BF00199792.
10
Human monoclonal antibodies.人源单克隆抗体。
Mol Cell Biochem. 1984 Jun;62(2):109-20. doi: 10.1007/BF00223301.

本文引用的文献

1
Human-human hybridomas producing monoclonal antibodies of predefined antigenic specificity.产生具有预定抗原特异性单克隆抗体的人-人杂交瘤。
Proc Natl Acad Sci U S A. 1980 Sep;77(9):5429-31. doi: 10.1073/pnas.77.9.5429.
2
Studies on specificity of cell-mediated immunity to human tumors.关于细胞介导的对人类肿瘤免疫特异性的研究。
J Natl Cancer Inst. 1974 Dec;53(6):1527-38.
3
A buffered chromic chloride method of attaching antigens to red cells: use in haemagglutination.一种将抗原附着于红细胞的缓冲氯化铬方法：用于血凝反应。
J Immunol Methods. 1974 May;5(1):91-6. doi: 10.1016/0022-1759(74)90050-7.
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Delayed-hypersensitivity reactions to soluble membrane antigens of human malignant lung cells.对人恶性肺细胞可溶性膜抗原的迟发型超敏反应。
J Natl Cancer Inst. 1974 Feb;52(2):327-38. doi: 10.1093/jnci/52.2.327.
5
Continuous cultures of fused cells secreting antibody of predefined specificity.分泌预定义特异性抗体的融合细胞的连续培养物。
Nature. 1975 Aug 7;256(5517):495-7. doi: 10.1038/256495a0.
6
In vitro assays of cell-mediated immunity to human solid tumors: problems of quantitation, specificity, and interpretation.
J Natl Cancer Inst. 1975 Oct;55(4):745-8. doi: 10.1093/jnci/55.4.745.
7
Further studies on a human lung tumor-associated antigen. Comparison of antigens from different tumors.关于一种人类肺肿瘤相关抗原的进一步研究。不同肿瘤抗原的比较。
J Biol Chem. 1979 Mar 25;254(6):2097-102.
8
A human thymus-leukemia antigen defined by hybridoma monoclonal antibodies.一种由杂交瘤单克隆抗体定义的人类胸腺白血病抗原。
Proc Natl Acad Sci U S A. 1979 Dec;76(12):6552-6. doi: 10.1073/pnas.76.12.6552.
9
Separation of functional subsets of human T cells by a monoclonal antibody.用单克隆抗体分离人T细胞的功能亚群
Proc Natl Acad Sci U S A. 1979 Aug;76(8):4061-5. doi: 10.1073/pnas.76.8.4061.
10
Cell surface antigens of human melanoma identified by monoclonal antibody.用单克隆抗体鉴定的人黑色素瘤细胞表面抗原
Proc Natl Acad Sci U S A. 1979 Jun;76(6):2927-31. doi: 10.1073/pnas.76.6.2927.