针对肺癌抗原的人源单克隆抗体。
Human monoclonal antibodies to lung-cancer antigens.
作者信息
Sikora K, Wright R
出版信息
Br J Cancer. 1981 May;43(5):696-700. doi: 10.1038/bjc.1981.101.
Abstract
Lymphocytes obtained from hilar and bronchial lymph nodes from 23 patients undergoing radical surgery for carcinoma of the bronchus were fused with established rat or mouse myeloma lines. 62% of the resultant hybrids were found to be secreting human Ig detected by a sensitive staphylococcal Protein A-coupled SRBC assay. Immunoglobulins synthesized by such hybrids were internally labelled with 3H-lysine and their antibody activity against a variety of membrane preparations determined. Nine monoclonal antibodies were found which bound to molecules on lung-cancer membranes and not on normal lung membranes from the same patient.
摘要
从23例接受支气管癌根治手术患者的肺门和支气管淋巴结中获取淋巴细胞，将其与已建立的大鼠或小鼠骨髓瘤细胞系进行融合。通过灵敏的葡萄球菌蛋白A偶联的绵羊红细胞试验检测发现，62%的所得杂交细胞分泌人免疫球蛋白。用3H-赖氨酸对这类杂交细胞合成的免疫球蛋白进行内标记，并测定其针对多种膜制剂的抗体活性。发现有9种单克隆抗体可与肺癌细胞膜上的分子结合，而不与同一患者正常肺细胞膜上的分子结合。
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本文引用的文献
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