Jensen G E, Clausen J
Ann Nutr Metab. 1981;25(1):27-37. doi: 10.1159/000176476.
Gluthathione peroxidase (EC 1.11.1.9, GSHPx) activity was measured in whole liver, in hepatic cytosol and in whole mitochondria from normal rats and from rats subjected to experimentally induced vitamin e and/or essential fatty acid (EFA) deficiency. The animals were fed and raised on the specific diets for 6 months. The GSHPx activity was significantly decreased in all samples of EFA-deficient rats. This group exhibited a significant increased 29:3omega9 content and to low 20:4omega6 content. In rats fed a vitamin E-deficient diet, the GSHP x activity was significantly decreased in hepatic cytosol. When the data of all experimental groups were pooled the specific GSHP x activities of the hepatic cytosol correlated significantly to that of whole mitochondria. The specific GSHP x activity of the whole liver, the mitochondrial and the cytosol fractions were found higher in female than in male rats in controls as well as in deficient animals. This difference was affected by the EFA deficiency. In EFA-deficient mitochondria the sex difference in enzymic activity was decreased (half of normal difference). In EFA-deficient cytosol the difference was increased (double of normal difference). However, the total hepatic GSHPx activities were not significantly higher in female than in male rats in any of the groups studied. Thus, EFA deficiency is added to the list of factors influencing the GSHPx activity. It is concluded that it is not possible to use GSHPx activity as a marker for the selenium status in domestic animals such as suggested previously. Thus, selenium is only one of the factors governing the GSHPx level.
在正常大鼠以及实验性诱导维生素E和/或必需脂肪酸（EFA）缺乏的大鼠的全肝、肝细胞溶胶和全线粒体中测量了谷胱甘肽过氧化物酶（EC 1.11.1.9，GSHPx）活性。这些动物在特定饮食下饲养6个月。EFA缺乏大鼠的所有样本中GSHPx活性均显著降低。该组29:3ω9含量显著增加，而20:4ω6含量较低。在喂食维生素E缺乏饮食的大鼠中，肝细胞溶胶中的GSHPx活性显著降低。当汇总所有实验组的数据时，肝细胞溶胶的特异性GSHPx活性与全线粒体的特异性GSHPx活性显著相关。在对照组以及缺乏营养的动物中，发现雌性大鼠全肝、线粒体和细胞溶胶部分的特异性GSHPx活性高于雄性大鼠。这种差异受EFA缺乏的影响。在EFA缺乏的线粒体中，酶活性的性别差异减小（为正常差异的一半）。在EFA缺乏的细胞溶胶中，差异增大（为正常差异的两倍）。然而，在任何研究组中，雌性大鼠的总肝GSHPx活性均未显著高于雄性大鼠。因此，EFA缺乏被添加到影响GSHPx活性的因素列表中。得出的结论是，如先前建议的那样，不可能将GSHPx活性用作家畜硒状态的标志物。因此，硒只是控制GSHPx水平的因素之一。
