Strong J E, Broughton A, Crooke S T
Cancer Treat Rep. 1977 Nov;61(8):1509-12.
The specificity of antisera produced in a goat or rabbit for use in bleomycin radioimmunoassay has been examined using competitive experiments employing several bleomycin analogs. These studies demonstrate that the antiserum distinguished alterations in both the nucleus and the terminal structure of the molecule. Removal of the terminal structure or addition of an aminosugar moiety in the bleomycin nucleus resulted in a significant decrease in immunoreactivity. Antisera produced in goat and rabbit hosts had different specificity for the terminal structure. The goat antisera demonstrated greater specificity for the terminal side chain than the rabbit antisera although this may not reflect a species-related event.
已通过使用几种博来霉素类似物的竞争性实验，对在山羊或兔子体内产生的用于博来霉素放射免疫分析的抗血清的特异性进行了检测。这些研究表明，该抗血清能够区分分子的核心结构和末端结构的变化。去除末端结构或在博来霉素核心中添加氨基糖部分会导致免疫反应性显著降低。在山羊和兔子宿主中产生的抗血清对末端结构具有不同的特异性。山羊抗血清对末端侧链的特异性高于兔子抗血清，尽管这可能并不反映与物种相关的情况。
