Berk R S, Iglewski B H, Hazlett L D
Infect Immun. 1981 Jul;33(1):90-4. doi: 10.1128/iai.33.1.90-94.1981.
We studied the responses of mice to ocular challenge with purified exotoxin A from Pseudomonas aeruginosa in 5-, 10-, 16-, 21-, and 30-day-old animals. In the absence of trauma, injection of 3 to 6 microgram of exotoxin per mouse beneath the fused eyelids of 5-day-old Swiss-Webster mice resulted in death of all animals within 24 h. Administration of 1.5, 0.75, and 0.375 microgram of exotoxin per mouse resulted in 24-h mortality rates of 50, 22, and 20%, respectively. Additional deaths were recorded throughout the next 4 days. Similar lethality results were obtained with 10-day-old animals that received equivalent amounts of exotoxin beneath the fused eyelid and in these experiments, the 72-h 50% lethal dose was 0.49 microgram of exotoxin per mouse. Mice that were 16 and 21 days old, whose eyelids were open, each received from 0.375 to 15 microgram of exotoxin topically applied to the surface of a wounded cornea. Cataracts were observed within 1 week in both groups, and none of the animals that received the higher concentrations of toxin died. Young adult (30-day-old) animals also received from 1.8 to 15 microgram of exotoxin topically on the surfaces of wounded corneas. Corneal swelling and slight opacity were observed at 24 h and within 1 month; 80% of these mice had cataracts of the ocular lens.
我们研究了5日龄、10日龄、16日龄、21日龄和30日龄小鼠对铜绿假单胞菌纯化外毒素A眼部攻击的反应。在无创伤情况下，给5日龄瑞士韦伯斯特小鼠融合眼睑下每只注射3至6微克外毒素，所有动物在24小时内死亡。每只小鼠注射1.5微克、0.75微克和0.375微克外毒素，24小时死亡率分别为50%、22%和20%。在接下来的4天内记录到更多死亡情况。给10日龄动物在融合眼睑下注射等量外毒素也得到类似的致死结果，在这些实验中，72小时50%致死剂量为每只小鼠0.49微克外毒素。16日龄和21日龄的小鼠眼睑已睁开，每只在受伤角膜表面局部应用0.375至15微克外毒素。两组在1周内均观察到白内障，接受较高毒素浓度的动物无一死亡。成年（30日龄）动物也在受伤角膜表面局部应用1.8至15微克外毒素。在24小时时观察到角膜肿胀和轻微浑浊，1个月内80%的这些小鼠出现晶状体白内障。
