利用微免疫珠（MIB-IgM）快速检测风疹特异性IgM抗体。

Rapid detection of rubella-specific IgM antibodies by the use of microimmunobeads (MIB-IgM).

作者信息

Braun R, Doerr H W, Horing C

出版信息

J Virol Methods. 1981 Jul;3(1):45-9. doi: 10.1016/0166-0934(81)90021-5.

Abstract

A simple haemagglutination (HA) titre reduction assay for the detection of rubella-specific IgM antibodies has been developed. Serum IgM is rapidly isolated by immune adsorption to heavy chain-specific anti-human IgM antibodies covalently coupled to polyacrylamide microimmunobeads. The immobilized immunocomplexes are incubated with rubella virus HA antigen. Subsequently, the particles are centrifuged from the solution, and the reduction of the HA titre is determined. This procedure has proven to be as reliable as haemagglutination inhibition (HAI) tests carried out with IgM fractions separated on a sucrose density gradient.

摘要

已开发出一种用于检测风疹特异性 IgM 抗体的简单血凝（HA）效价降低试验。血清 IgM 通过免疫吸附到与聚丙烯酰胺微免疫珠共价偶联的重链特异性抗人 IgM 抗体上而快速分离。将固定化的免疫复合物与风疹病毒 HA 抗原一起孵育。随后，从溶液中离心分离颗粒，并测定 HA 效价的降低情况。该方法已被证明与用在蔗糖密度梯度上分离的 IgM 组分进行的血凝抑制（HAI）试验一样可靠。