Dufrain R J, Littlefield L G, Wilmer J L
Mutat Res. 1980 Jan;69(1):101-5. doi: 10.1016/0027-5107(80)90179-7.
Cytogenetic analyses of cultured blood lymphocytes from streptonigrin-treated female rabbits demonstrated that the yield of chemically-induced chromatid aberrations, expecially exchanges, is influenced by the method of blood handling before in vitro culture. When blood cells from treated animals were washed with tissue-culture medium before culture, chromatid breaks were observed at the same level as in the controls, and no chromatid exchanges were found. However, when duplicate aliquots of blood were inoculated directly into culture medium, without washing, elevated levels of chromatid breaks were observed and chromatid exchanges were found. These findings indicate that the clastogen, streptonigrin, was carried into the culture medium with the blood-cell inoculum.
对经链黑菌素处理的雌性兔子的培养血淋巴细胞进行的细胞遗传学分析表明，化学诱导的染色单体畸变（尤其是交换）的产生受体外培养前血液处理方法的影响。当处理动物的血细胞在培养前用组织培养基洗涤时，观察到的染色单体断裂水平与对照组相同，且未发现染色单体交换。然而，当将两份等量血液直接接种到培养基中而不进行洗涤时，观察到染色单体断裂水平升高且发现了染色单体交换。这些发现表明，诱变剂链黑菌素随血细胞接种物带入了培养基中。
