Ito N, Shirai T, Fukushima S, Hirose M
J Cancer Res Clin Oncol. 1984;108(1):169-73. doi: 10.1007/BF00390992.
A dose-response study was carried out in male F344 rats with a specific urinary bladder carcinogen, N-butyl-N-(4-hydroxybutyl)nitrosamine (BBN), which was administered as a solution in drinking water for up to 112 weeks. BBN was given in four different concentrations, 50, 10, 5, and 1 ppm, to groups of 30 rats. The highest dose induced urinary bladder cancer in all rats treated by week 91, decreasing yields being associated with lower dose levels: an incidence of 76.7% was observed with 10 ppm, followed by 20.0% with 5 ppm. The lowest dose (1 ppm) only induced two cases of papilloma (6.9%).
对雄性F344大鼠进行了一项剂量反应研究，使用一种特定的膀胱致癌物N-丁基-N-(4-羟基丁基)亚硝胺（BBN），将其配制成饮用水溶液，给药长达112周。将BBN以50、10、5和1 ppm四种不同浓度给予每组30只大鼠。最高剂量在第91周时使所有接受治疗的大鼠发生膀胱癌，随着剂量水平降低，发病率也随之降低：10 ppm时观察到的发病率为76.7%，其次是5 ppm时为20.0%。最低剂量（1 ppm）仅诱发了两例乳头状瘤（6.9%）。
