Masui T
J Embryol Exp Morphol. 1981 Apr;62:277-89.
To reveal differentiation potency of yolk-sac endoderm, this tissue from quail embryos was cultured alone or in association with digestive-tract mesenchymes of chick embryos. When yolk-sac endoderm was cultured alone in vitro, the endoderm of the area vitellina differentiated into the yolk-sac parenchyma, but the endoderm of the extraembryonic area pellucida (EEAP) failed to differentiate into yolk-sac parenchyma, and the endoderm of the area vasculosa became necrotic. When endoderm of the area vitellina was cultured in association with digestive-tract mesenchymes, all the endodermal cells developed into yolk-sac parenchymal cells after two days. Later, basophilic cells appeared among them, and differentiated into both mesenchyme-specific epithelia and intestinal-type epithelium with a striated border, and villi were also formed. Goblet cells appeared in all types of recombinations. The endoderm of the EEAP cultured with digestive-tract mesenchymes gave similar results to that of the area vitellina. In contrast, endoderm of the area vasculosa, when cultured with digestive-tract mesenchymes, became necrotic. The present investigation demonstrated that the endoderms of the area vitellina and of the EEAP differ in self-differentiation potency, and that their developmental fates can be modified by the influence of digestive-tract mesenchymes. These endoderms can differentiate into the mesenchyme-specific epithelia, though they often differentiate also into the intestinal-type epithelium.
为了揭示卵黄囊内胚层的分化潜能，将鹌鹑胚胎的该组织单独培养或与鸡胚的消化道间充质联合培养。当卵黄囊内胚层在体外单独培养时，卵黄区的内胚层分化为卵黄囊实质，但胚外透明区（EEAP）的内胚层未能分化为卵黄囊实质，血管区的内胚层则发生坏死。当卵黄区内胚层与消化道间充质联合培养时，所有内胚层细胞在两天后都发育为卵黄囊实质细胞。随后，其中出现嗜碱性细胞，并分化为间充质特异性上皮和具有纹状缘的肠型上皮，还形成了绒毛。杯状细胞出现在所有类型的重组中。与消化道间充质联合培养的EEAP内胚层产生了与卵黄区相似的结果。相比之下，血管区内胚层与消化道间充质联合培养时则发生坏死。本研究表明，卵黄区和EEAP的内胚层在自我分化潜能上存在差异，并且它们的发育命运可因消化道间充质的影响而改变。这些内胚层虽常分化为肠型上皮，但也能分化为间充质特异性上皮。
