Yasugi Sadao, Kedinger Michèle, Simon-Assmann Patricia, Bouziges Françoise, Haffen Katy
Zoological Institute, Faculty of Science, University of Tokyo, Hongo, 113, Tokyo, Japan.
Unite 61 INSERM, Biologie cellulaire et physiopathologie digestive, 3 Avenue Molière, 67200, Strasbourg-Hautepierre, France.
Rouxs Arch Dev Biol. 1989 Jun;198(2):114-117. doi: 10.1007/BF02447746.
Proventricular epithelium (PV epithelium) from 6-day chicken embryos was associated with cultured cells, derived from fetal rat small intestine, or with fetal rat or human skin fibroblasts. The cytodifferentiation of PV epithelium was investigated using antibodies to chicken pepsinogen, a marker protein of PV epithelium, and to chicken sucrase, a marker enzyme of the small-intestinal brush-border membrane.PV epithelium formed complex glands and produced pepsinogen in association with cultured gut mesenchymal cells and skin fibroblasts. Its development was comparable to that achieved under the influence of PV mesenchyme. PV epithelial development was severely inhibited, however, under the influence of intact chicken or rat intestinal mesenchyme. The data are consistent with the idea that during the first step of epithelial-mesenchymal interactions, the epithelium and not the mesenchyme may be responsible for the determination of the developmental fate.
来自6日龄鸡胚的前胃上皮（PV上皮）与源自胎鼠小肠的培养细胞、胎鼠或人皮肤成纤维细胞相关联。使用针对鸡胃蛋白酶原（PV上皮的一种标记蛋白）和鸡蔗糖酶（小肠刷状缘膜的一种标记酶）的抗体，研究了PV上皮的细胞分化。PV上皮与培养的肠道间充质细胞和皮肤成纤维细胞一起形成复杂的腺体并产生胃蛋白酶原。其发育与在PV间充质影响下所达到的发育情况相当。然而，在完整的鸡或大鼠肠道间充质的影响下，PV上皮发育受到严重抑制。这些数据与以下观点一致，即在上皮-间充质相互作用的第一步中，决定发育命运的可能是上皮而不是间充质。
