带有I-J决定簇的抑制性T细胞的特异性富集：平行的功能和血清学特征分析
Specific enrichment of the suppressor T cell bearing I-J determinants: parallel functional and serological characterizations.
作者信息
Okumura K, Takemori T, Tokuhisa T, Tada T
出版信息
J Exp Med. 1977 Nov 1;146(5):1234-45. doi: 10.1084/jem.146.5.1234.
Abstract
A simple procedure to enrich the antigen (keyhole limpet hemocyanin, KLH)-specific suppressor T cell was described. The suppressor T cell from KLH)-immunized mice specifically bound to the KLH-coated Sephadex G-200 column at 37 degrees C, and was eluted from the column by cold (0-4 degrees C) medium. The helper T cell did not bind to the column under the identical condition. The suppressor T cell thus obtained had 100 times as potent suppressor activity as the original spleen cells in in vivo and in vitro secondary antibody responses against a hapten coupled to KLH. This procedure also enriched the cells bearing I-J determinants and Lyt-2,3 alloantigens, allowing us to study the phenotypic expressions on the suppressor T cell by direct serological procedures as well as by the use of the fluorescence activated cell sorter. Parallel functional and serological analyses indicated that the antigen-specific suppressor T cell belongs to a population of I-J+, Lyt-2+,3+ and Fc R- T cells.
摘要
本文描述了一种富集抗原（钥孔戚血蓝蛋白，KLH）特异性抑制性T细胞的简单方法。来自经KLH免疫小鼠的抑制性T细胞在37℃时特异性结合于包被KLH的葡聚糖凝胶G - 200柱，并通过冷（0 - 4℃）培养基从柱上洗脱下来。在相同条件下，辅助性T细胞不与该柱结合。如此获得的抑制性T细胞在体内和体外针对与KLH偶联的半抗原的二次抗体应答中，其抑制活性比原始脾细胞强100倍。该方法还富集了携带I - J决定簇和Lyt - 2、3同种抗原的细胞，这使我们能够通过直接血清学方法以及使用荧光激活细胞分选仪来研究抑制性T细胞的表型表达。平行的功能和血清学分析表明，抗原特异性抑制性T细胞属于I - J +、Lyt - 2 +、3 +和Fc R - T细胞群体。
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Specific enrichment of the suppressor T cell bearing I-J determinants: parallel functional and serological characterizations.带有I-J决定簇的抑制性T细胞的特异性富集：平行的功能和血清学特征分析
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2
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