Ureña V, Garcia R, Vela C, Ortiz F, Lahoz C
Int Arch Allergy Appl Immunol. 1979;59(2):135-42. doi: 10.1159/000232251.
Antigenic stimulation of peripheral lymphocytes was made in two different groups of atopic patients, testing their capabilities to produce IgE and IgG in vitro. For this purpose, Marbrook's technique was used in the cultures. The immunoglobulins produced were measured in the supernatants by a sensitive double-antibody method. Results demonstrate that it is possible to produce enough IgE and IgG to be detected. Some important parameters had to be considered: appropriate selection of the patients and the antigens used in the antigenic stimulation, as well as the number of cells and the duration of the culture.
在两组不同的特应性患者中对外周淋巴细胞进行抗原刺激，测试其体外产生IgE和IgG的能力。为此，在培养中采用了马尔布鲁克技术。通过灵敏的双抗体法测定上清液中产生的免疫球蛋白。结果表明，可以产生足够量的可被检测到的IgE和IgG。必须考虑一些重要参数：患者的适当选择、抗原刺激中使用的抗原、细胞数量以及培养持续时间。
