Akanuma H, Ogawa K, Lee Y, Akanuma Y
J Biochem. 1981 Jul;90(1):157-62. doi: 10.1093/oxfordjournals.jbchem.a133445.
Anhydroglucitol in human plasma was studied by high pressure liquid chromatography, which was employed to simplify sampling procedures for the polyol assay and to avoid enzymatic treatment of the plasma for removal of glucose. Polyols in the column effluent were assayed by a flow fluorometric method, which involved two continuous successive reactions; periodate oxidation and the Hantzsch reaction. Plasma, 1 ml each, from 55 diabetic and 18 control subjects was deproteinized, concentrated, and subjected to the liquid chromatography. The results of this analysis indicated that the plasma anhydroglucitol level showed an inverse correlation with the plasma glucose level, while the correlation of glycerol and glucose levels was obscure.
采用高压液相色谱法对人血浆中的1,5 - 脱水葡萄糖醇进行了研究，该方法用于简化多元醇检测的采样程序，并避免对血浆进行酶处理以去除葡萄糖。采用流动荧光法对柱流出物中的多元醇进行检测，该方法涉及两个连续的反应：高碘酸盐氧化反应和汉茨希反应。分别从55名糖尿病患者和18名对照受试者中采集1ml血浆，进行去蛋白、浓缩处理后，再进行液相色谱分析。分析结果表明，血浆1,5 - 脱水葡萄糖醇水平与血浆葡萄糖水平呈负相关，而甘油水平与葡萄糖水平之间的相关性不明显。
