Dopheide T A, Ward C W
J Gen Virol. 1981 Feb;52(Pt 2):367-70. doi: 10.1099/0022-1317-52-2-367.
The site of bromelain cleavage in the haemagglutinin of the Hong Kong influenza virus A/Memphis/102/72 has been determined by using a diagonal peptide mapping procedure on the thermolytic digest of amidated BHA. The data show that bromelain cleavage removes the C-terminal 46 residues from HA2, and that the new carboxyl-terminal residue of BHA2 is Gly 175. This is close to the beginning of the hydrophobic membrane-interacting sequence that starts at residue 183.
通过对酰胺化BHA的热解消化产物进行对角线肽图谱分析，已确定香港甲型流感病毒A/孟菲斯/102/72血凝素中菠萝蛋白酶切割位点。数据表明，菠萝蛋白酶切割从HA2去除了C端的46个残基，且BHA2的新羧基末端残基是Gly 175。这接近始于第183位残基的疏水膜相互作用序列的起始处。
