聚（dG-dC）左右手性形式之间的转变
The transitions between left- and right-handed forms of poly(dG-dC).
作者信息
Ivanov V I, Minyat E E
出版信息
Nucleic Acids Res. 1981 Sep 25;9(18):4783-98. doi: 10.1093/nar/9.18.4783.
Abstract
The circular dichroism study of water/trifluoroethanol (TFE) solutions of poly(dG-dC) has revealed the following: The polynucleotide is present as a B form up to a TFE content of 60% (v/v) or less. Then, a cooperative transition into a left-handed Z form occurs. Within the region of 66-78% TFE, a continuous non-cooperative change is going on which can be attributed to an intrafamily transition within the family of Z forms. At last, in the interval of 80-84% TFE, a second cooperative transition, probably, Z - A is realized. Both transitions, Z - A and Z - B, show slow kinetics (10-60 min) while the direct transitions from the A to B form taking less than 10 sec. The length of cooperativity for the B - Z transition, Vo = 25 base pairs was estimated using spermine molecules. Spermine was found to induce the B to Z transition in the (dG-dC) sequences even in the absence of TFE which might be biologically interesting.
摘要
聚（dG-dC）的水/三氟乙醇（TFE）溶液的圆二色性研究揭示了以下内容：在TFE含量达到60%（v/v）或更低时，多核苷酸以B型存在。然后，会发生协同转变为左手Z型。在66 - 78% TFE区域内，正在进行连续的非协同变化，这可归因于Z型家族内部的家族内转变。最后，在80 - 84% TFE区间内，可能实现了第二次协同转变，即Z - A转变。Z - A和Z - B这两种转变都显示出缓慢的动力学（10 - 60分钟），而从A到B型的直接转变用时不到10秒。使用精胺分子估计了B - Z转变的协同长度，Vo = 25个碱基对。发现精胺即使在没有TFE的情况下也能在（dG-dC）序列中诱导B到Z的转变，这可能具有生物学意义。
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本文引用的文献
1
High-salt d(CpGpCpG), a left-handed Z' DNA double helix.高盐条件下的d（CpGpCpG），一种左手Z'型DNA双螺旋结构。
Nature. 1980 Aug 7;286(5773):567-73. doi: 10.1038/286567a0.
2
Left-handed DNA helices.左手DNA螺旋
Nature. 1980 Feb 21;283(5749):743-5. doi: 10.1038/283743a0.
3
Left-handed deoxyribonucleic acid double helix in solution.溶液中的左手螺旋脱氧核糖核酸双螺旋
Biochemistry. 1981 Mar 31;20(7):2036-41. doi: 10.1021/bi00510a046.
4
Salt-induced co-operative conformational change of a synthetic DNA: equilibrium and kinetic studies with poly (dG-dC).盐诱导的合成DNA的协同构象变化：聚（dG-dC）的平衡和动力学研究
J Mol Biol. 1972 Jun 28;67(3):375-96. doi: 10.1016/0022-2836(72)90457-3.
5
The geometry of nucleic acids.核酸的几何学
Prog Biophys Mol Biol. 1970;21:265-319. doi: 10.1016/0079-6107(70)90027-1.
6
Different conformations of double-stranded nucleic acid in solution as revealed by circular dichroism.圆二色光谱揭示溶液中双链核酸的不同构象。
Biopolymers. 1973;12(1):89-110. doi: 10.1002/bip.1973.360120109.
7
Effect of detergents on photoinduced changes of absorption of Chromatium minutissimum chromatophores.洗涤剂对极小色杆菌色素细胞光诱导吸收变化的影响。
Mol Biol. 1972 Sep-Oct;6(5):624-31.
8
Conformational changes in DNA molecules.DNA分子中的构象变化。
Annu Rev Biophys Bioeng. 1974;3(0):127-50. doi: 10.1146/annurev.bb.03.060174.001015.
9
Conformation and reactivity of DNA. VI. Circular dichroism studies of salt-induced conformational changes of DNAs of different base composition.DNA的构象与反应性。VI. 盐诱导不同碱基组成DNA构象变化的圆二色性研究。
Biochim Biophys Acta. 1974 Aug 15;361(1):11-32.
10
Optical studies of a conformational change in DNA before melting.DNA解链前构象变化的光学研究。
J Mol Biol. 1972 Apr 14;65(3):381-99. doi: 10.1016/0022-2836(72)90196-9.
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