Meloan S N, Puchtler H
Histochemistry. 1978 Dec 1;58(3):163-6. doi: 10.1007/BF00495715.
Previous histochemical investigations demonstrated similarities in the binding of Congo Red and other direct cotton dyes by amyloid and cellulose. It seemed therefore of interest to determine whether or not the cellulose-like reactivity of amyloid extends also to dye solutions containing an anionic reserving agent. These reagents are used in the dyeing of wool-cellulose (Halbwolle) fabrics to prevent binding of direct cotton dyes by proteins. Mesitol WLS-Congo Red solutions stained amyloid selectively; other tissue structures, except some hyaline deposits in arterioles, remained unstained. The cause of this non-specific reaction could not be determined with certainty. Therefore, the alkaline Congo Red method is recommended for histochemical identification of amyloid. However, the Mesitol WLS-Congo Red technic was very useful for demonstration of amyloid after prolonged storage of tissues in formalin; amyloid in such material showed little or no reactivity with the alkaline Congo Red or the Sirius dye methods. This pilot study indicates that anionic reserving agents can be effectively employed under conditions of histochemical technics.
以往的组织化学研究表明，淀粉样蛋白和纤维素对刚果红及其他直接棉染料的结合具有相似性。因此，确定淀粉样蛋白的纤维素样反应性是否也适用于含有阴离子匀染剂的染料溶液似乎很有意义。这些试剂用于羊毛 - 纤维素（半毛）织物染色，以防止直接棉染料与蛋白质结合。间甲酚WLS - 刚果红溶液可选择性地染色淀粉样蛋白；其他组织结构，除小动脉中的一些透明沉积物外，均未染色。这种非特异性反应的原因尚不能确定。因此，推荐使用碱性刚果红法进行淀粉样蛋白的组织化学鉴定。然而，间甲酚WLS - 刚果红技术对于在福尔马林中长时间保存的组织中淀粉样蛋白的显示非常有用；此类材料中的淀粉样蛋白对碱性刚果红或天狼星染料法几乎没有或没有反应。这项初步研究表明，阴离子匀染剂可在组织化学技术条件下有效应用。
