Ogata K
Nihon Seikeigeka Gakkai Zasshi. 1981 Aug;55(8):769-77.
Since the first description by Aukland and co-authors in 1964, the hydrogen washout has been shown to be an accurate method in determining regional tissue blood flow. The presence of hydrogen molecules within the tissue is detected with a platinum electrode where a small amount of current is generated by oxidation of molecular hydrogen to hydrogen ions. Therefore, construction of the suitable electrode for the tissue to be measured is essential. The author applied the hydrogen washout technique to the blood flow measurement of bone, muscle, skin, digit and peripheral nerve, and found that the technique was valuable in basic and clinical studies in orthopedics. As a typical experimental study using the hydrogen washout technique, the study on the effect of adrenaline on bone blood flow was presented and the experimental method was explained in detail. Although the hydrogen washout technique has been developed to measure the blood flow, the technique has been found useful in detecting the pathways of microcirculation between different tissues. As an example, the study on nutritional pathways of the intervertebral disk was described. Since the hydrogen gas is harmless, it is possible to apply the technique to the clinical studies including the blood flow measurement of replanted digits, diagnosis of the compartment syndrome and the blood flow measurement of skin flaps. Furthermore, several problems in the hydrogen washout technique were discussed.
自1964年奥克兰及其合著者首次描述以来，氢洗脱已被证明是确定局部组织血流的一种准确方法。组织内氢分子的存在通过铂电极进行检测，在该电极处，分子氢氧化成氢离子会产生少量电流。因此，构建适合待测组织的电极至关重要。作者将氢洗脱技术应用于骨骼、肌肉、皮肤、手指和周围神经的血流测量，发现该技术在骨科基础研究和临床研究中具有重要价值。作为使用氢洗脱技术的典型实验研究，展示了肾上腺素对骨血流影响的研究，并详细解释了实验方法。尽管氢洗脱技术已被开发用于测量血流，但该技术在检测不同组织之间的微循环通路方面也很有用。例如，描述了关于椎间盘营养通路的研究。由于氢气无害，因此可以将该技术应用于包括再植手指血流测量、骨筋膜室综合征诊断和皮瓣血流测量在内的临床研究。此外，还讨论了氢洗脱技术中的几个问题。
