Cohen B J
J Med Virol. 1978;3(2):141-9. doi: 10.1002/jmv.1890030208.
Little is known about the immunoglobulin class of antibodies to HBcAg. In the present study sera containing anti-HBc were fractionated by surcose density-gradient centrifugation, and all serum fractions were tested against HBcAg by immunoelectro-osmophoresis. In addition selected fractions were examined by complement fixation test, immune adherence hemagglutination and immune electron microscopy. Anti-HBc activity in IgG serum fractions was demonstrated by all four techniques used, but HBcAg-specific IgM was detected only by immunoelectro-osmophoresis and by immune electron microscopy. In acute hepatitis B, HBcAg-specific IgM was detected for up to eight weeks after the onset of jaundice. It was also found transiently in two patients who developed chronic hepatitis B without an icteric episode and in one out of thirteen patients with HBsAg-positive chronic liver disease, but in none of eight healthy HBsAg carriers. The results suggested that HBcAg-specific IgM is formed transiently in response to primary HBV infection but is generally undetectable in established HBsAg carriers.
关于针对乙肝核心抗原（HBcAg）的抗体的免疫球蛋白类别，人们了解甚少。在本研究中，通过蔗糖密度梯度离心法对含有抗-HBc的血清进行分级分离，并用免疫电渗电泳法对所有血清级分进行HBcAg检测。此外，还通过补体结合试验、免疫黏附血凝试验和免疫电子显微镜检查对选定的级分进行了检测。所用的四种技术均证实了IgG血清级分中存在抗-HBc活性，但仅通过免疫电渗电泳法和免疫电子显微镜检测到了HBcAg特异性IgM。在急性乙型肝炎中，黄疸出现后长达八周均可检测到HBcAg特异性IgM。在两名未出现黄疸发作而发展为慢性乙型肝炎的患者以及13名HBsAg阳性慢性肝病患者中的1名患者中也短暂检测到了该物质，但在8名健康的HBsAg携带者中均未检测到。结果表明，HBcAg特异性IgM是在原发性HBV感染后短暂形成的，但在已确诊的HBsAg携带者中通常无法检测到。
