Varela-Díaz V M, Coltorti E A, Rickard M D, Torres J M
Res Vet Sci. 1977 Sep;23(2):213-6.
Hydatid cyst fluid from Echinococcus granulosus (HCF) and cyst fluid from Taenia hydatigena (TCF) cysts were compared in reciprocal immunoelectrophoresis (IEP) tests using homologous and heterologous antisera which were free of antibodies to host serum contaminants. The antigens for the E granulosus arc 5 were demonstrated in TCF. Antibody activity to these and other antigens common to HCF and TCF was removed from homologous antisera by absorptions with the heterologous antigenic preparation. Antigens not shared by the two metacestodes fluids were then demonstrated by IEP tests. These findings are discussed in terms of their significance to phylogenetic and immunodiagnostic studies of these parasites in their immediate hosts.
在双向免疫电泳(IEP)试验中,使用不含针对宿主血清污染物抗体的同源和异源抗血清,对细粒棘球绦虫的棘球蚴囊液(HCF)和泡状带绦虫囊肿液(TCF)进行了比较。在TCF中证实了细粒棘球绦虫弧5的抗原。通过用异源抗体制剂吸收,从同源抗血清中去除了针对这些以及HCF和TCF共有的其他抗原的抗体活性。然后通过IEP试验证实了两种中绦期液体不共有的抗原。根据这些发现对这些寄生虫在其直接宿主中的系统发育和免疫诊断研究的意义进行了讨论。