Oliver R E, Gorham J R, Perryman L E, Spencer G R
Am J Vet Res. 1981 Sep;42(9):1560-4.
Three groups of 4 lambs each were experimentally infected with an isolate of progressive pneumonia virus (PPV) by intracerebral, intra-articular, or intrathoracic routes of inoculation. An additional control group was sham-inoculated by all 3 routes with identically prepared, noninfected inoculum. Sheep were killed 12 to 42 days after inoculation. A mild generalized leptomeningitis was found in 2 of the 4 lambs inoculated intracerebrally. A severe, proliferative synovitis with massive infiltration of lymphocytes and plasma cells was found in the 4 lambs inoculated intraarticularly. Germinal center formation was found in the synovial membrane 42 days after inoculation. Appreciable differences in lung morphologic features were not observed among the 4 groups. The PPV was isolated from virus-inoculated sheep from the choroid plexus, synovium, and lungs from inoculated and noninoculated sites. Nine of the 12 virus-inoculated lambs developed serum antibody to PPV, and specific antibodies were found in the synovial fluid of virus-inoculated joints. Virus was not isolated, lesions were not found, and serum antibody was not demonstrated in sham-inoculated sheep.
将4只羔羊分为三组，分别通过脑内、关节内或胸腔内接种途径，用进行性肺炎病毒（PPV）的一种分离株进行实验性感染。另外设立一个对照组，通过所有这三种途径用同样制备的未感染接种物进行假接种。接种后12至42天处死绵羊。在4只脑内接种的羔羊中，有2只出现轻度全身性软脑膜炎。在4只关节内接种的羔羊中，发现严重的增生性滑膜炎，伴有淋巴细胞和浆细胞大量浸润。接种42天后，在滑膜中发现生发中心形成。4组之间未观察到肺形态特征有明显差异。从接种病毒的绵羊的脉络丛、滑膜以及接种和未接种部位的肺中分离出PPV。12只接种病毒的羔羊中有9只产生了针对PPV的血清抗体，并且在接种病毒的关节的滑液中发现了特异性抗体。在假接种的绵羊中未分离到病毒，未发现病变，也未检测到血清抗体。
