Ritchie D G, Fuller G M
Inflammation. 1981 Dec;5(4):275-87. doi: 10.1007/BF00911093.
Primary cultures of adult rat hepatocytes were used to assay for the presence of leukocytic mediator(s) (LEM), a neutrophil derived protein(s) capable of stimulating the synthesis of acute-phase plasma proteins when injected into rats. In the presence of physiological concentrations of dexamethasone (40 mM), the hepatocytes secreted a variety of plasma proteins as demonstrated by crossed immunoelectrophoresis. The addition of LEM to hepatocytes increased the secretion of several acute-phase related plasma proteins, including fibrinogen and hepatoglobin, and decreased albumin secretion. These results mimic the acute-phase response observed in the intact animal. Fibrinogen secretion was used as a quantitative marker for determining LEM activity. The rate of fibrinogen secretion depended upon both the concentration of dexamethasone and LEM present during a given 24-h assay period. One unit of LEM activity is defined as that concentration of LEM capable of producing a 50% maximal stimulation of fibrinogen secretion.
成年大鼠肝细胞的原代培养物用于检测白细胞介质（LEM）的存在，LEM是一种从中性粒细胞衍生的蛋白质，当注入大鼠体内时能够刺激急性期血浆蛋白的合成。在生理浓度的地塞米松（40 mM）存在下，通过交叉免疫电泳证明肝细胞分泌多种血浆蛋白。向肝细胞中添加LEM增加了几种急性期相关血浆蛋白的分泌，包括纤维蛋白原和肝珠蛋白，并减少了白蛋白分泌。这些结果模拟了在完整动物中观察到的急性期反应。纤维蛋白原分泌用作确定LEM活性的定量标志物。纤维蛋白原分泌速率取决于给定24小时检测期内存在的地塞米松和LEM的浓度。一个单位的LEM活性定义为能够产生50%最大纤维蛋白原分泌刺激的LEM浓度。
