Woloski B M, Fuller G M
Proc Natl Acad Sci U S A. 1985 Mar;82(5):1443-7. doi: 10.1073/pnas.82.5.1443.
Leukemia cell lines of the monocytic series (HL-60, U-937, and P388D1) produce a hepatocyte-stimulating factor (HSF) following induction of differentiation with phorbol diester. In 24-72 hr, these leukemia cells produce 2-30% the amount of HSF as human peripheral blood monocytes. Cells of the series at earlier stages of differentiation produced greater amounts of HSF. Fractionation of the medium from each cell type by HPLC reveals much of the HSF activity in the 25- to 30-kilodalton range. Under the same culture conditions, interleukin 1 is produced; however, its bioactivity is in the 7- to 15-kilodalton range. Neither monokine shows reciprocal bioactivity. Superinducing culture conditions that greatly increase interleukin 1 production completely eliminate HSF production, suggesting that there is different stability of the mRNA coding for each protein or that there are different temporal events important to the induction of synthesis of these proteins.
单核细胞系白血病细胞株（HL - 60、U - 937和P388D1）在用佛波酯诱导分化后会产生一种肝细胞刺激因子（HSF）。在24至72小时内这些白血病细胞产生的HSF量为人外周血单核细胞产生量的2%至30%。该细胞系中处于分化早期阶段的细胞产生的HSF量更多。通过高效液相色谱法对每种细胞类型的培养基进行分级分离，发现大部分HSF活性处于25至30千道尔顿范围内。在相同培养条件下会产生白细胞介素1；然而其生物活性处于7至15千道尔顿范围内。这两种单核因子均未表现出相互的生物活性。极大增加白细胞介素1产生量的超诱导培养条件会完全消除HSF的产生，这表明编码每种蛋白质的mRNA稳定性不同，或者对于这些蛋白质合成的诱导存在不同的时间事件。
