Vecchini A, Binaglia L, Palmerini C A, Floridi A, Porcellati G
Ital J Biochem. 1981 Sep-Oct;30(5):367-74.
The uptake of labelled cytidine from primary cell cultures from rat embryo brain has been studied. A preferential flow of the cytosine moiety towards CDP-choline is evident. Labelled uracyl derivatives represent only a small fraction of the radioactivity measured in the hydrosoluble fraction of the cell extract, at any incubation time. CDP-choline synthesized from labelled cytidine belongs to the metabolic pool implicated in phospholipid synthesis. The evidence for the uptake of the intact cytidine molecule is also given.
对来自大鼠胚胎脑的原代细胞培养物中标记胞苷的摄取进行了研究。胞嘧啶部分向CDP-胆碱的优先流动是明显的。在任何孵育时间,标记的尿嘧啶衍生物仅占细胞提取物水溶性部分中测得放射性的一小部分。由标记胞苷合成的CDP-胆碱属于参与磷脂合成的代谢池。同时也给出了完整胞苷分子摄取的证据。