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用于透射电子显微镜检查的从细胞学涂片制备物中去除选定细胞的方法。

Method for the removal of selected cells from cytological smear preparations for transmission electron microscopy.

作者信息

Mather J, Stanbridge C M, Butler E B

出版信息

J Clin Pathol. 1981 Dec;34(12):1355-7. doi: 10.1136/jcp.34.12.1355.

DOI:10.1136/jcp.34.12.1355
PMID:7328181
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC494599/
Abstract

The method described depends on fixing additional cytology smear preparations with glutaraldehyde to be used, if needed. Removal of cells from the slide makes use of partially polymerised resins in the usual way but a paper marker is used to localise the area in the resin block which contains the cells to be examined. Slides are placed in a freezing cabinet to facilitate easy separation of the resin block.

摘要

所述方法取决于用戊二醛固定额外的细胞学涂片制备物，以便在需要时使用。以常规方式利用部分聚合的树脂从载玻片上移除细胞，但使用纸质标记物来定位树脂块中包含待检查细胞的区域。将载玻片放入冷冻柜中，以便于树脂块的轻松分离。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3315/494599/e4e3bbcd7270/jclinpath00493-0046-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3315/494599/c77b8f9964e3/jclinpath00493-0045-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3315/494599/e4e3bbcd7270/jclinpath00493-0046-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3315/494599/c77b8f9964e3/jclinpath00493-0045-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3315/494599/e4e3bbcd7270/jclinpath00493-0046-a.jpg

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An Alternative Approach for Sample Preparation with Low Cell Number for TEM Analysis.一种用于低细胞数量样本制备以进行透射电子显微镜分析的替代方法。
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An efficient and reproducible process for transmission electron microscopy (TEM) of rare cell populations.一种高效且可重现的用于透射电子显微镜（TEM）稀有细胞群体成像的方法。
J Immunol Methods. 2014 Feb;404:87-90. doi: 10.1016/j.jim.2013.11.025. Epub 2013 Dec 1.

本文引用的文献

1
Virus identification in cytologic and histologic material by electron microscopy.
Acta Cytol. 1969 Apr;13(4):206-9.
2
The method of selective detection of exfoliated vaginal cells in the electron microscope.电子显微镜下阴道脱落细胞的选择性检测方法。
Acta Cytol. 1967 Nov-Dec;11(6):437-8.
3
Ultrastructure of normal epithelial cells in Papanicolaou-stained cervical smears. An application of a modified open-face embedding technique for transmission electron microscopy.巴氏染色宫颈涂片中正常上皮细胞的超微结构。一种用于透射电子显微镜的改良开放式包埋技术的应用。
Acta Cytol. 1979 Nov-Dec;23(6):507-15.
4
Human papovavirus in Papanicolaou smears of urinary sediment detected by transmission electron microscopy.
J Clin Pathol. 1977 Nov;30(11):1015-20. doi: 10.1136/jcp.30.11.1015.