一种改良的乙肝表面抗原微凝集技术与标准技术及一种新的放射免疫分析系统的比较。
Comparison of a modified microagglutination technique for HBsAg with the standard technique and a new RIA system.
作者信息
Supran E M, Gardner P S, Cayzer I, Court G, Chalmers S
出版信息
J Clin Pathol. 1981 Dec;34(12):1396-9. doi: 10.1136/jcp.34.12.1396.
A further modification of the standard RPHA technique (Hepatest, Wellcome Reagents) for the detection of HBsAg is described. This modification does not require a centrifugation step which is required by the other modifications that have been described previously and consequently takes a little longer to perform. It does, however, retain the advantages of increased sensitivity and decreased costs which are also features of the other modifications. A series of 939 routine clinical specimens were used to evaluate the method described and to evaluate a new RIA kit for the detection of HBsAg (Hepatube, Wellcome Reagents). Of 53 specimens found to be positive for HBsAg by RIA, 50 (94% were detected by the modified Hepatest RPHA as opposed to 47 (89%) by the standard technique.
本文描述了一种用于检测乙肝表面抗原（HBsAg）的标准反向被动血凝试验（RPHA）技术（惠康试剂公司的Hepatest）的进一步改良方法。这种改良方法不需要像之前描述的其他改良方法那样进行离心步骤，因此操作所需时间稍长一些。然而，它确实保留了提高灵敏度和降低成本的优点，这也是其他改良方法的特点。使用一系列939份常规临床标本对所描述的方法进行评估，并评估一种用于检测HBsAg的新型放射免疫分析试剂盒（惠康试剂公司的Hepatube）。在通过放射免疫分析发现的53份HBsAg阳性标本中，改良后的Hepatest RPHA检测出50份（94%），而标准技术检测出47份（89%）。
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