Paulson D J, Light K E
Res Commun Chem Pathol Pharmacol. 1981 Sep;33(3):559-62.
Experimental diabetes was induced in rats by IV administration of 65 mg/kg streptozotocin. After 12 days, the rats were anesthetized with ether; the hearts were excise and perfusion washed. The atria and basilar portion of ventricle were freeze clamped, weighed and assayed for content of norepinephrine (NE), epinephrine (E) and dopamine (DA) using high pressure liquid chromatography with electrochemical detection. The serum concentration and adrenal content of NE, E and DA were also assayed. Results showed a significant increase in NE and DA in diabetic rat ventricles compared to control. The serum concentration of NE was significantly increased in diabetics while E showed no change.
通过静脉注射65毫克/千克链脲佐菌素诱导大鼠患实验性糖尿病。12天后，用乙醚麻醉大鼠；取出心脏并进行灌注冲洗。将心房和心室基部冷冻钳夹、称重，并使用带电化学检测的高压液相色谱法测定去甲肾上腺素（NE）、肾上腺素（E）和多巴胺（DA）的含量。还测定了血清中NE、E和DA的浓度以及肾上腺中的含量。结果显示，与对照组相比，糖尿病大鼠心室中的NE和DA显著增加。糖尿病大鼠血清中NE的浓度显著升高，而E无变化。
