从兽医临床样本中鉴定凝固酶阳性中间葡萄球菌和猪葡萄球菌猪葡萄球菌亚种。
Identification of coagulase-positive Staphylococcus intermedius and Staphylococcus hyicus subsp. hyicus isolates from veterinary clinical specimens.
作者信息
Phillips W E, Kloos W E
出版信息
J Clin Microbiol. 1981 Dec;14(6):671-3. doi: 10.1128/jcm.14.6.671-673.1981.
Abstract
Coagulase-positive isolates of Staphylococcus intermedius from dogs and coagulase-positive isolates of Staphylococcus hyicus subsp. hyicus from a pig and cows were identified initially by a simplified scheme which can be readily performed in a routine diagnostic laboratory. Characters tested in the scheme included coagulase activity, colony pigment, and aerobic acid production from maltose. The identity of these isolates was confirmed by deoxyribonucleic acid-deoxyribonucleic acid hybridization experiments. The strains of S. hyicus subsp. hyicus isolated from porcine sources were positive for protein A, whereas the strains recovered from bovine mastitic milk were negative for protein A.
摘要
最初通过一种可在常规诊断实验室轻松开展的简化方法，鉴定了来自犬的中间葡萄球菌凝固酶阳性分离株以及来自猪和牛的猪葡萄球菌猪亚种凝固酶阳性分离株。该方法检测的特征包括凝固酶活性、菌落色素以及麦芽糖的需氧酸生成情况。通过脱氧核糖核酸-脱氧核糖核酸杂交实验证实了这些分离株的身份。从猪源分离的猪葡萄球菌猪亚种菌株蛋白A呈阳性，而从牛乳腺炎乳汁中分离的菌株蛋白A呈阴性。
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