Peña C, Stewart J M, Ferrara P, Paladini A C
Int J Pept Protein Res. 1981 Sep;18(3):289-96. doi: 10.1111/j.1399-3011.1981.tb02983.x.
The solid phase synthesis of fragments 75-120-NH2 and 73-128-GlyNH2 of the human growth hormone sequence is described. Purification was by solvent extraction, gel filtration and partition chromatography. No growth-promoting activity was detected in any of the peptides by the rat body-growth assay. An immunochemically reactive area was located in the sequence 73-128 when tested by complement fixation assays.
描述了人生长激素序列片段75 - 120 - NH₂和73 - 128 - GlyNH₂的固相合成。通过溶剂萃取、凝胶过滤和分配色谱法进行纯化。通过大鼠身体生长试验在任何一种肽中均未检测到生长促进活性。当通过补体结合试验进行检测时，在序列73 - 128中定位到一个免疫化学反应区域。
