人成纤维细胞干扰素的氨基末端氨基酸序列
NH2-terminal amino acid sequence of human fibroblast interferon.
作者信息
Stein S, Kenny C, Friesen H J, Shively J, Del Valle U, Pestka S
出版信息
Proc Natl Acad Sci U S A. 1980 Oct;77(10):5716-9. doi: 10.1073/pnas.77.10.5716.
Abstract
The purification of human fibroblast interferon by chromatography on Blue Sepharose and high-performance liquid chromatography is described. The amino acid composition and a partial sequence of the homogeneous protein are reported. The NH2 terminus was determined to be NH2-Met1-Ser-Tyr-Asn-Leu-Leu-Gly-Phe-Leu-Gln-Arg-Ser-Ser-Asn-Phe-Gln-X-Gln-Lys.
摘要
本文描述了通过蓝色琼脂糖凝胶柱色谱法和高效液相色谱法纯化人成纤维细胞干扰素的过程。报告了该纯蛋白的氨基酸组成和部分序列。确定其氨基末端为NH2-Met1-Ser-Tyr-Asn-Leu-Leu-Gly-Phe-Leu-Gln-Arg-Ser-Ser-Asn-Phe-Gln-X-Gln-Lys。
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