Use of the elaborated multiple exposure photography (MEP) method in routine sperm motility analyses and for research purposes.

Elaboration of the three basic components incorporated in the recently developed multiple exposure photography (MEP) method for objective sperm motility determination is described. This includes an improved version of the 10-micrometers counting chamber, modified procedures of photography, and a system for easier calculation of the results. During the last 2 years several thousands of sperm motility analyses were performed with the elaborated technique. In our institute this method has totally replaced motility evaluation by subjective estimation and has become the sole method for semen assessment performed during routine clinical work-up and research studies. This method is compared with other still-camera photographic techniques, and data about duration of performance, details of the relatively low cost of analysis, and specifications of some of its components are presented as well.