Shieh H S, Berman H M, Dabrow M, Neidle S
Nucleic Acids Res. 1980 Jan 11;8(1):85-97. doi: 10.1093/nar/8.1.85.
A 2:2 complex of proflavine and deoxycytidylyl-3', 5'-guanosine has been crystallized and its structure determined by x-ray crystallography. The two dinucleoside phosphate strands form self complementary duplexes with Watson Crick hydrogen bonds. One proflavin is asymmetrically intercalated between the base pairs and the other is stacked above them. The conformations of the nucleotides are unusual in that one strand has C3',C2'endomixed sugar puckering and the other has C3',C3' endo deoxyribose sugars. These results show that the conformation of the 3'sugar is of secondary importance to the intercalated geometry.
硫酸原黄素与脱氧胞苷酰-3',5'-鸟苷的2:2复合物已结晶,并通过X射线晶体学确定了其结构。两条二核苷磷酸链通过沃森-克里克氢键形成自我互补的双链体。一个硫酸原黄素不对称地插入碱基对之间,另一个则堆积在它们之上。核苷酸的构象不同寻常,因为一条链具有C3',C2'内向混合糖折叠,另一条链具有C3',C3'内向脱氧核糖糖。这些结果表明,3'-糖的构象对插入几何形状的重要性次之。