Huff V, Littlefield L G, DuFrain R J, Wilmer J L
Cytogenet Cell Genet. 1981;30(1):15-24. doi: 10.1159/000131583.
In previous cytogenetic studies of rabbits injected with the clastogen, streptonigrin (SN), we observed a higher incidence of chromosome aberrations in lymph node cells than in marrow cells recovered 6 h after treatment. In this study we examined several factors that may be responsible for this difference in the incidence of lesions. Results from SN pulse-treated lymphoid and marrow cultures demonstrated that the responses were different in vitro also. In addition, at higher doses of SN, lymphoid cells displayed a greater sensitivity to the drug when treated during DNA synthesis; marrow cells showed no differences in sensitivity throughout the cell cycle. Analysis of the percentage of lymph node and marrow metaphases labeled following 2, 4, or 6 h of culture in the presence of tritiated thymidine indicated that G2 was less than 2 h in lymph node cells and approximately 4 hr in marrow cells. From these data we conclude that the difference in the incidence of lesions 6 hr after an in vivo SN exposure. However, our data also indicate that lymph node cells are innately more sensitive to the induction of lesions than are marrow cells.
在之前对注射了断裂剂链黑菌素（SN）的兔子进行的细胞遗传学研究中，我们观察到，在处理后6小时回收的淋巴结细胞中，染色体畸变的发生率高于骨髓细胞。在本研究中，我们考察了可能导致这种损伤发生率差异的几个因素。对经SN脉冲处理的淋巴细胞和骨髓细胞培养物的研究结果表明，体外反应也存在差异。此外，在较高剂量的SN作用下，淋巴细胞在DNA合成期间接受处理时对该药物表现出更高的敏感性；骨髓细胞在整个细胞周期中敏感性没有差异。对在含有氚标记胸腺嘧啶核苷的情况下培养2、4或6小时后标记的淋巴结和骨髓中期细胞百分比的分析表明，淋巴结细胞的G2期少于2小时，而骨髓细胞约为4小时。从这些数据我们得出结论，体内暴露于SN 6小时后损伤发生率存在差异。然而，我们的数据还表明，淋巴结细胞天生就比骨髓细胞对损伤诱导更敏感。
