Bailey D N, Guba J J
Clin Chem. 1980 Mar;26(3):437-40.
We describe a reliable gas-chromatographic analysis for unchanged phencyclidine in human plasma, with use of a nitrogen detector. The assay is sufficiently sensitive to permit measurement of as little as 5 microgram of the drug per liter, in 2 mL of plasma. Phencyclidine is extracted from plasma at pH 10.5 into hexane/iso-amyl alcohol containing meperidine as the internal standard, back-extracted into dilute HCl, and re-extracted into hexane/iso-amyl alcohol after alkalinization of the HCl. The coefficients of variation for the analysis at respective concentrations of 100 and 200 microgram/L are: within-run 4.0% and 3.4%; between-run, 2.8% and 5.2%. This procedure, used in our laboratory for more than one year, has been applied to plasma from numerous individuals with nonfatal phencyclidine intoxication. Some representative examples are given.
我们描述了一种利用氮检测器对人血浆中未变化的苯环己哌啶进行可靠气相色谱分析的方法。该测定法灵敏度足够高，能够在2毫升血浆中检测到低至每升5微克的药物。苯环己哌啶在pH值为10.5的条件下从血浆中萃取到含有哌替啶作为内标的己烷/异戊醇中，再反萃取到稀盐酸中，盐酸碱化后又重新萃取到己烷/异戊醇中。在100微克/升和200微克/升各自浓度下分析的变异系数为：批内分别为4.0%和3.4%；批间分别为2.8%和5.2%。该程序在我们实验室已使用一年多，已应用于众多非致命性苯环己哌啶中毒个体的血浆检测。文中给出了一些代表性示例。
