总状毛霉高频异核体的形成
High-frequency heterokaryon formation by Mucor racemosus.
作者信息
Lasker B A, Borgia P T
出版信息
J Bacteriol. 1980 Feb;141(2):565-9. doi: 10.1128/jb.141.2.565-569.1980.
Abstract
Heterokaryons from Mucor racemosus were produced from two auxotrophic strains of the fungus. Germlings were converted to spheroplasts by using commercial chitinase and purified Myxobacter AL-1 chitosanase. Spheroplasts from the auxotrophic strains were mixed and fused in solutions of polyethylene glycol and CaCl2. Under optimal conditions, prototrophic heterokaryons were formed at a frequency of about 5%.
摘要
来自总状毛霉的异核体是由该真菌的两个营养缺陷型菌株产生的。通过使用商业几丁质酶和纯化的粘细菌AL-1壳聚糖酶，将芽管转化为原生质球。将营养缺陷型菌株的原生质球在聚乙二醇和氯化钙溶液中混合并融合。在最佳条件下，原养型异核体以约5%的频率形成。
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