从猪垂体中分离促脂解素的C片段和C'片段以及从脑中分离C片段。
Isolation of the C-fragment and C'-fragment of lipotropin from pig pituitary and C-fragment from brain.
作者信息
Smyth D G, Snell C R, Massey D E
出版信息
Biochem J. 1978 Oct 1;175(1):261-70. doi: 10.1042/bj1750261.
Abstract
Three novel peptides derived from lipotropin, the C-Fragment (residues 61-91), C'-Fragment (61-87) and N-Fragment (1-38), were isolated from pig pituitary, and the C-Fragment was shown to be present in brain. The experimental procedures developed for their isolation are described. The formation of each of the fragments involves enzymic cleavage of lipotropin at consecutive basic residues, with specificity identical with that involved in the activation of known prohormones. In brain assays C-Fragment exhibits a range of biological activities related to its ability to act as an inhibitory neurotransmitter.
摘要
从猪垂体中分离出了三种源自促脂素的新型肽，即C片段（第61 - 91位氨基酸残基）、C'片段（61 - 87位）和N片段（1 - 38位），并且发现C片段存在于大脑中。文中描述了为分离这些肽而开发的实验步骤。每个片段的形成都涉及促脂素在连续碱性残基处的酶促裂解，其特异性与已知激素原激活过程中涉及的特异性相同。在大脑检测中，C片段表现出一系列与其作为抑制性神经递质的作用能力相关的生物活性。
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