人黑色素瘤异种移植瘤的体外化学敏感性试验
In vitro chemosensitivity tests on xenografted human melanomas.
作者信息
Bateman A E, Selby P J, Steel G G, Towse G D
出版信息
Br J Cancer. 1980 Feb;41(2):189-98. doi: 10.1038/bjc.1980.29.
Abstract
An in vitro chemosensitivity test has been applied to malignant melanoma cells from 5 patients. The tumour cells were first grown as xenografts in immune-suppressed mice, so that the results of the in vitro test could be compared with precise measurements of the sensitivity of the melanoma cells when exposed to chemotherapeutic drugs in vivo in the mouse. The in vitro assay involved exposing the tumour cells to each of 8 drugs, after which cell survival was determined by colony assay in soft agar. Dose-response curves were obtained and the surviving fraction at drug levels estimated to be achieved in man was used as a measure of in vitro drug sensitivity. Significant differences among the 8 drugs were detected, and these accorded with clinical experience. The correlation of in vivo (in the mouse) and in vitro sensitivities to Melphalan and MeCCNU was also significant.
摘要
已对5例患者的恶性黑色素瘤细胞进行了体外化学敏感性试验。肿瘤细胞首先作为异种移植物在免疫抑制小鼠体内生长，以便将体外试验结果与黑色素瘤细胞在小鼠体内接受化疗药物时的敏感性精确测量结果进行比较。体外试验包括将肿瘤细胞暴露于8种药物中的每一种，之后通过软琼脂集落试验确定细胞存活率。获得了剂量反应曲线，并将估计在人体中可达到的药物水平下的存活分数用作体外药物敏感性的指标。检测到8种药物之间存在显著差异，且这些差异与临床经验相符。美法仑和甲基环己亚硝脲的体内（在小鼠体内）和体外敏感性之间的相关性也很显著。
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